Semiconductor Laser-Induced Fluorescence Detection/Capillary Electrophoresis.
نویسندگان
چکیده
منابع مشابه
High-sensitivity laser-induced fluorescence detection for capillary electrophoresis.
Capillary electrophoresis provides very high efficiency separations of biological molecules. Laser-induced fluorescence produces very high sensitivity detection. The combination of the two techniques results in an analytical tool with unprecedented properties: separations with more than a million theoretical plates and detection limits of a few hundred analyte molecules. This paper considers th...
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Capillary electrophoresis (CE) enables rapid separations with high separation efficiency and compatibility with small sample volumes. Laser-induced fluorescence detection can result in extremely low limits of detection in CE. Single-channel fluorescence detection, however, furnishes little qualitative information about a species being detected, except for its CE migration time. Use of multidime...
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Antibody (Ab) labeled with alkaline phosphatase was separated and detected by a capillary electrophoresis system equipped with a laser induced fluorescence detection system. The combination of B/F separation and the LIF detection of the label enzyme is potentially useful for a highly sensitive detection of antigen (Ag). After B/F separation by capillary electrophoresis (CE), label enzymes catal...
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CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo...
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The first application of the violet diode laser for laser induced fluorescence (LIF) detection in capillary electrophoresis is reported. Operating at 405 nm with an output power of 5 mW, this laser offers many advantages over conventional red diode lasers operating at 635 nm. We demonstrate the suitability of the violet diode laser for LIF detection by detecting amino acids labeled with naphtha...
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ژورنال
عنوان ژورنال: Journal of the Spectroscopical Society of Japan
سال: 1995
ISSN: 1884-6785,0038-7002
DOI: 10.5111/bunkou.44.21